Domain-specific Gene Activation by Parathyroid Hormone in Osteoblastic ROS17/2.8 Cells

摘要: Parathyroid hormone (PTH)-mediated gene activation was assessed in the osteoblast-like rat cell line ROS17/2.8 with two PTH fragments harboring distinct activating domains: PTH-(1-34) and PTH-(28-48). The response of genes expressed immediate early cycle or osteoblast developmental sequence investigated. In addition, subtractive cloning used to identify cells that are activated by domains. immediately increased transcript levels c-fos c-jun at a considerably higher rate than A significant effect on osteoblastic marker could not be detected, exception elevated ornithine decarboxylase levels. However, continuous application osteoblast-specific osteocalcin reduced those other including alkaline phosphatase PTH/PTH-related peptide receptor. By cloning, nine cDNAs were isolated corresponding mRNAs directly up-regulated Among these cyclic phosphodiesterase, (cytosine 5)-methyltransferase, an 80-kDa protein kinase C substrate, junB, novel GC-binding protein. Three unknown present. Interestingly, all cases, efficiency PTH-(28-48) substantially lower comparison PTH-(1-34). PTH-mediated signaling may therefore constitute minor pathway dominant cAMP/protein cascade.