Genetic variability of the V1 and V2env domains of SIVcpz-ant and neutralization pattern of plasma viruses in a chimpanzee infected naturally

作者: Pascale Ondoa , David Davis , Betty Willems , Leo Heyndrickx , Luc Kestens

DOI: 10.1002/JMV.2102

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摘要: Laboratory of Viral Pathogenesis, Biomedical Primate Research Center, Rijswijk, The NetherlandsSpecific neutralizing epitope changes havebeenobservedinachimpanzeeinfectednaturallywith SIVcpz, which differ from HIV-1 infectinghumans.Tocharacterizefurtherthesechanges,alongitudinal study env genomic sequencevariation SIVcpz-ant isolates was undertakenin this animal. V1 and V2 regions the envwere determined to arise specific recombi-nationevents.Todeterminewhetherrecombina-tion domains possiblyassociated with emergence neutralizationescape viruses, envelope sequences genelength polymorphisms PBMC plasmaviral variants were studied over a 7-year period.PBMCs plasma-associated infectious virustiters as well plasma RNA viral loads weremonitored longitudinally. first 5 virusesisolated found beneutralization escape variants. Sequence analy-sis their indicated that a20 amino acid stretch region hadundergone recombination also asso-ciated elicitingstrong neutralization responses. These findingssupportthehypothesisthatrecombinationoftheV1 play role inneutralization SIVcpz in chimpanzeesinfectednaturally. Furthermore, data confirmthat antibody response plays animportantroleinthedeclineofplasmainfectiousvirustitersinHIV-1relatedSIVcpznonpathogenicinfection. J.Med.Virol.65:765–776,2001.

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