The development of a real-time biosensor for the detection of trace levels of trinitrotoluene (TNT) in aquatic environments
作者： E.S. Bromage , T. Lackie , M.A. Unger , J. Ye , S.L. Kaattari
DOI: 10.1016/J.BIOS.2006.10.001
关键词:
摘要: Abstract This paper describes the development of a highly sensitive TNT immunosensor consisting specific monoclonal antibody coupled with prototype fluorescence-based detector system (KinExA Inline Biosensor, Sapidyne Instrument Inc). The developed possesses high affinity for (association constant, aK 8.2) minimal cross reactivity other compounds such as tetryl, 2,4-dinitrotoluene and 2-amino-4,6-dinitrotoluene. provides sample assessment within 160 s from source acquisition sensitivity 0.05 μg/L in ground water. sensor can be regenerated 8 min, allows minimum 40 repeated readings, has standard error 0.1–0.4% between repeat readings. fluidics software allow samples to obtained up eight different sources allowing user examine stratification pollutant water column. We believe that this used rapidly assess trace levels environmental samples.
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