Human colostral cells. I. Separation and characterization.

摘要: Analyses of the cells present in human colostrum obtained from fifty-four healthy donors during first four days lactation revealed that there were 3.3 x 10(6) (range 1.1 10(5)--1.2 10(7)) per ml colostrum. Based on histochemical examinations, it was found this population consisted 30--47% macrophages, 40--60% polymorphonuclear leucocytes, 5.2--8.9% lymphocytes, and 1.3--2.8% colostral corpuscles; epithelial rarely encountered. The identity various cell types confirmed by Wright's stain a series techniques which disclosed presence non-specific esterase, peroxidase, lipids. For further characterization, different separated methods, such as Ficoll-Hypaque density centrifugation, isokinetic centrifugation linear Ficoll gradient, adherence to glass or plastic, phagocytosis carbonyl iron. Immunohistochemical staining with FITC- and/or TRITC-labelled reagents IgA, IgM, IgG, K- lambda-chains, secretory component, lactoferrin, alpha-lactalbumin applied unseparated well cells. Polymorphonuclear leucocytes (staining for peroxidase) macrophages corpuscles esterase) exhibited numerous intracellular vesicles contained lipids combinations milk proteins. Lymphoid did not any these plasma detected among Individual phagocytic immunoglobulins IgA IgM classes, both K lambda light chains, alpha-lactalbumin. coincidental appearance proteins single, but lymphoid indicate acquired ingestion environment. Markers commonly used identification B lymphocytes (surface immunoglobulins) T (receptors sheep red blood cells) unreliable analysis (unless accompanied subsequent morphological characterization) because strong fluorescence observed surface many non-lymphoid formed rosettes (SRBC). Lymphocytes, often association corpuscles, classified