Propranolol reduces anoxia/reoxygenation-mediated injury of adult myocytes through an anti-radical mechanism

摘要: The effects of propranolol (PRO) and atenolol (ATE) on adult canine myocytes exposed to 30 min anoxia (A:95% N2/5% CO2) subsequent reoxygenation (R:95% O2/5% for up 20 was investigated. In some studies, comparison were made with that superoxide dismutase (SOD). Although alone produced only minimal injury, in the absence beta-blockers or SOD associated significant losses cellular viability, elevated release lactate dehydrogenase, increased formation lipid peroxidation products. Myocytes A/R presence d,1-PRO (20, 200 microM) afforded substantial, concentration-dependent protection during reoxygenation. Significant also observed 2 microM d-PRO (non-active beta-blocker), but after a longer preincubation period (2 h). (10 micrograms/ml) provided equi-potent d,1-PRO. By contrast, more water-soluble beta-blocker, ATE (200 microM), offered minor protection. Electron Spin Resonance spin trapping studies using alpha-phenyl-tert-butylnitrone (PBN) performed drug treatment. Short-term min) exposure prior A/R, SOD, resulted 71-84% reduction total PBN radical adduct (alkoxyl; alpha H = 2.0-2.5 G, N 13.5-13.75 G); long-term h) 51% reduction. These data suggest anion an initiator events leading anti-peroxidative properties PRO appear be independent beta-receptor blockade.