Cypermethrin, chlorpyrifos, deltamethrin, and imidacloprid exposure up-regulates the mRNA and protein levels of bdnf and c-fos in the brain of adult zebrafish (Danio rerio).

作者: Selçuk Özdemir , Serdar Altun , Mustafa Özkaraca , Atena Ghosi , Emine Toraman

DOI: 10.1016/J.CHEMOSPHERE.2018.03.190

关键词:

摘要: The aim of the present study is to investigate toxicity effects frequently used pesticides, involving cypermethrin, deltamethrin, chlorpyrifos and imidacloprid, on expression bdnf c-fos genes in zebrafish brain tissues. Therefore, tissues exposed intoxication was primarily analyzed by indirect immunofluorescence assay. Afterwards, mRNA transcription levels BNDF protein were measured qRT-PCR Western blotting, respectively. data assay revealed intensive immunopositivity for pesticide comparison control group (p<0.05). Moreover, genes, elevated following (p<0.05, p<0.01, p<0.001, respectively). These results showed that exposure acute imidacloprid disrupted normal neuronal activity, resulting neurotoxic effect, also DNA-binding Increasing activation, an oncoprotein from family Nuclear Proteins, true knowledge these chemicals are oncogenic Thus, use pesticides poses a potential risk non-target organisms.

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