Genetic Diversity, Phylogenetics and Molecular Systematics of Guizotia Cass. (Asteraceae)

摘要: The genus Guizotia belongs to the tribe Heliantheae in family Asteraceae. It has been placed under different subtribes. its center of origin, distribution and genetic diversity Ethiopia, where G. abyssinica (niger) domesticated. Amplified Fragment Length Polymorphism (AFLP), Random Polymorphic DNA (RAPD) sequencing were applied study diversity, phylogenetics, molecular systematics this genus. A large number niger populations, representing all regions Ethiopia crop is grown, was investigated using AFLP RAPD marker techniques. extent variation distributed throughout growing regions, regardless altitude cultivation. Despite fact that most within significant population differentiation obtained (AMOVA; P < 0.001) guizotias. concluded both wild and/or weedy relatives have wide bases need be conserved utilized for improvement abyssinica. Further collection germplasm exploration conservation highly localized guizotias are recommended. Most diagnostic markers generated from AFLPs RAPDs specific arborescens zavattarii. Phylogenetic analyses undertaken based on sequence data internal transcribed spacers (ITS) five chloroplast regions. revealed a close phylogenetic relationship between scabra ssp. schimperi support previous suggestion latter progenitor former. According study, scabra, schimperi, Chelelu Ketcha populations best viewed at present as separate species Guizotia. Those perennial with geographic appears evolved first during evolutionary history This supports placement subtribe Milleriinae. suggested composition subtribal redefined.