Molecular detection and treatment of tick-borne pathogens in domestic dogs in Khon Kaen, northeastern Thailand.

摘要: We determined the prevalence of tick-borne pathogens in domestic dogs using microscopy and polymerase chain reaction (PCR) techniques. A total 303 EDTA blood samples were collected from Khon Kaen Province, Thailand, May 2013. Microscopic observation Giemsa-stained smears molecular diagnosis conventional PCR performed. Infected treated with imidocarb dipropionate, a combination dipropionate doxycycline, or doxycycline alone. Seventy-one (23.4%) out positive for DNA pathogens. Of animals, 13.2% 1.3% single infection Babesia spp Ehrlichia canis, respec- tively microscopy; whereas 19.5% 3.0% technique. Co-infection E. canis was observed 0.7%, co-infection Hepatozoon 0.3%. assigned drugs, elimination demonstrated by PCR. The results indicated that while both microscopic diagnostic techniques useful pathogen detection, more effective. Imidocarb found to be effec - tive treatment babesiosis ehrlichiosis, respectively. present study suggests technique has high sensitivity specificity as well detection spp, H. specimens.