Selective binding of chicken progesterone receptor a subunit to a DNA fragment containing ovalbumin gene sequences

摘要: Summary Progesterone receptor A protein, purified to near-homogeneity from laying hen oviducts, binds preferentially a cloned fragment of the chicken ovalbumin gene containing portion transcribed sequences, including transcriptional start site, as well extensive sequences preceding 5′ end gene. As revealed using nitrocellulose filter adsorption assay isolate receptor-DNA complexes, non-specific interaction with DNA is characterized by selective binding largest fragments present in mixtures bacterial plasmid DNA. In contrast, smaller bound mixture larger fragment. Furthermore, selectivity for retained even presence six-fold molar excess nonradioactive analogous region adult β-globin Direct competition studies and globin 5′-DNA show at least ten-fold preference hormone-responsive