Acellular and Cellular Lung Model to Study Tumor Metastasis

摘要: It is difficult to isolate tumor cells at different points of progression. We created an ex vivo lung model that can show the interaction with a natural matrix and continual flow nutrients, as well shows normal cellular components matrix. The acellular by isolating rat heart-lung block removing all using decellularization process. right main bronchus tied off are placed in trachea syringe. move populate left lung. then bioreactor where pulmonary artery receives media closed circuit. grown on primary tumor. isolated circulating metastatic lesions. skipping Each be used answer research questions.