Identification of a Highly Conserved Surface on Tat Variants
作者： Sonia Mediouni , Albert Darque , Isabelle Ravaux , Gilbert Baillat , Christian Devaux
关键词:
摘要: Extracellular Tat is suspected to protect HIV-1-infected cells from cellular immunity. Seropositive patients are unable produce neutralizing antibodies against Tat, and still secreted under antiviral treatment. In mice, the OYI vaccine candidate generates such as mAb 7G12. A peptide called MIMOOX was designed fragments of identified possible binding site for chemically synthesized, its structure stabilized with a disulfide bridge. Circular dichroism spectra showed that had mainly β turns but no α helix OYI. recognized by 7G12 in ELISA only reduced conditions. Moreover, competitive recognition assay between variants mimics highly conserved surface variants. Rat immunizations induce recognizing main HIV-1 subtypes confirm approach.
highwire.org参考文章(45)
S.E. Koken, A.E. Greijer, K. Verhoef, J. van Wamel, A.G. Bukrinskaya, B. Berkhout, Intracellular analysis of in vitro modified HIV Tat protein. Journal of Biological Chemistry. ,vol. 269, pp. 8366- 8375 ,(1994) , 10.1016/S0021-9258(17)37203-4
Barbara Ensoli, Giuseppe Barbanti-Brodano, Monica Betti, Chiara Boarini, Elisabetta Caselli, Antonella Caputo, Aurelio Cafaro, Pier Giorgio Balboni, Maria Pia Grossi, Cristina Rossi, DNA immunization with HIV-1 tat mutated in the trans activation domain induces humoral and cellular immune responses against wild-type Tat. Journal of Immunology. ,vol. 162, pp. 5631- 5638 ,(1999)
Solène Debaisieux, Fabienne Rayne, Hocine Yezid, Bruno Beaumelle, The ins and outs of HIV-1 Tat. Traffic. ,vol. 13, pp. 355- 363 ,(2012) , 10.1111/J.1600-0854.2011.01286.X
Michael O. Westendorp, Rainer Frank, Christina Ochsenbauer, Kirstin Stricker, Jens Dhein, Henning Walczak, Klaus-Michael Debating, Peter H. Krammer, Sensitization of T cells to CD95-mediated apoptosis by HIV-1 Tat and gp120. Nature. ,vol. 375, pp. 497- 500 ,(1995) , 10.1038/375497A0
Sonia Mediouni, Jennifer D. Watkins, Michel Pierres, Angélique Bole, Erwann P. Loret, Gilbert Baillat, A Monoclonal Antibody Directed against a Conformational Epitope of the HIV-1 Trans-activator (Tat) Protein Neutralizes Cross-clade Journal of Biological Chemistry. ,vol. 287, pp. 11942- 11950 ,(2012) , 10.1074/JBC.M111.319863
HÅVARD VALVATNE, ANNE MARIE SZILVAY, DAG E. HELLAND, A monoclonal antibody defines a novel HIV type 1 Tat domain involved in trans-cellular trans-activation AIDS Research and Human Retroviruses. ,vol. 12, pp. 611- 619 ,(1996) , 10.1089/AID.1996.12.611
Sonia Mediouni, Albert Darque, Gilbert Baillat, Isabelle Ravaux, Catherine Dhiver, Hervé Tissot-Dupont, Malika Mokhtari, Hervé Moreau, Catherine Tamalet, Corinne Brunet, Pascale Paul, Francoise Dignat-George, Andreas Stein, Philippe Brouqui, Stephen A Spector, Grant R Campbell, Erwann P Loret, None, Antiretroviral Therapy Does Not Block the Secretion of the Human Immunodeficiency Virus Tat Protein Infectious disorders drug targets. ,vol. 12, pp. 81- 86 ,(2012) , 10.2174/187152612798994939
Catherine Grégoire, Jean-Marie Péloponèse, Didier Esquieu, Sandrine Opi, Grant Campbell, Murielle Solomiac, Evelyne Lebrun, Jacques Lebreton, Erwann P. Loret, Homonuclear (1)H-NMR assignment and structural characterization of human immunodeficiency virus type 1 Tat Mal protein. Biopolymers. ,vol. 62, pp. 324- 335 ,(2001) , 10.1002/BIP.10000
Alain Lecoq, Gervaise Moine, Laurent Bellanger, Pascal Drevet, Robert Thai, Evelyne Lajeunesse, André Ménez, Michel Léonetti, Increasing the humoral immunogenic properties of the HIV-1 Tat protein using a ligand-stabilizing strategy. Vaccine. ,vol. 26, pp. 2615- 2626 ,(2008) , 10.1016/J.VACCINE.2008.02.057
B. C. Bachler, M. Humbert, B. Palikuqi, N. B. Siddappa, S. K. Lakhashe, R. A. Rasmussen, R. M. Ruprecht, Novel Biopanning Strategy To Identify Epitopes Associated with Vaccine Protection Journal of Virology. ,vol. 87, pp. 4403- 4416 ,(2013) , 10.1128/JVI.02888-12