XFEL data analysis for structural biology

作者: Haiguang Liu , John C. H. Spence

DOI: 10.1007/S40484-016-0076-Z

关键词:

摘要: X-ray Free Electron Lasers (XFELs) have advanced research in structure biology, by exploiting their ultra-short and bright pulses. The resulting “diffraction before destruction” experimental approach allows data collection to outrun radiation damage, a crucial factor that has often limited resolution the determination of biological molecules. Since first hard laser (the Linac Coherent Light Source (LCLS) at SLAC) commenced operation 2009, serial femtosecond crystallography (SFX) rapidly matured into method for structural analysis nano- micro-crystals. At same time, single particle coherent diffractive imaging, with one (such as virus) per shot, been under intense development. In this review we describe these applications lasers focus particularly on aspects computational community.We summarize key problems model reconstruction, provide perspectives future using methods.

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