RNA modifications detection by comparative Nanopore direct RNA sequencing

摘要: RNA molecules undergo a vast array of chemical post-transcriptional modifications (PTMs) that can affect their structure and interaction properties. To date, over 150 naturally occurring PTMs have been identified, however the overwhelming majority functions remain elusive. In recent years, small number successfully mapped to transcriptome using experimental approaches relying on high-throughput sequencing. Oxford Nanopore direct-RNA sequencing (DRS) technology has shown be sensitive modifications. We developed validated Nanocompore, robust analytical framework evaluate presence in DRS data. do so, we compare an sample interest against non-modified control sample. Our strategy does not require training set allows use replicates model biological variability. Here, demonstrate ability Nanocompore detect at single-molecule resolution human polyA+ RNAs, as well targeted non-coding RNAs. results correlate with orthogonal methods, confirm previous observations distribution N6-methyladenosine sites provide novel insights into coding transcriptomes. The latest version obtained https://github.com/tleonardi/nanocompore/