Efficient adsorption of tumor necrosis factor with an in vitro set-up of the microspheres-based detoxification system.

摘要: Background: The Microsperes-Based Detoxification System (MDS) represents a flexible therapeutic option for various diseases, depending on the specificity of adsorbents applied. A potential application MDS is supportive therapy sepsis. Methods: Microadsorbents tumor necrosis factor (TNF) were prepared by immobilization anti-TNF antibodies cellulose (1–10 µm) and applied in an experimental set-up using pool human plasma (1 liter) spiked with TNF (800 pg/ml) its soluble receptors (1,000 pg/ml each). Removal was compared filter albumin-permeable Albuflow. Results: Addition 4 (2) g adsorbent to filtrate circuit reduced concentrations 80% (64%). rates did not differ significantly different filters. adsorption influenced presence excess receptors. Conclusions: Concentrations mediators can be efficiently modulated small quantities specific adsorbents.