Purification and identification of a glycoprotein that induces the attachment of oncomiracidia of Neobenedenia girellae (Monogenea, Capsalidae)

摘要: Neobenedenia girellae, a monogenean skin parasite, shows low host specificity. N. girellae is an important pathogen in marine cultured fish such as yellowtail and amberjack. An effective control method required but none has yet been established. To clarify the mechanisms of specificity, we purified identified attachment-inducing substances oncomiracidia from tiger puffer fish. The were mainly included mucous extract. Skin extract lost its ability to induce attachment after boiling and/or exposure reducing agent dithiothreitol, suggesting that are proteinaceous nature. Since lectins Con A, WGA, PHA-L, PSA inhibited induction attachment, proteins suspected be glycoproteins. Glycoproteins specifically interacting with A collected by anion exchange chromatography, resulting two active peaks (peaks 3-A 6). component peak was Wap 65-2 N-terminal amino acid sequencing, while glycoprotein 6 could not identified. These results suggested recognised another their host.