Detection of nucleic acids by target-catalyzed product formation

摘要: A method is disclosed for modifying an oligonucleotide, which has application to the detection of a polynucleotide analyte. An oligonucleotide reversibly hybridized with polynucleotide, example, analyte, in presence 5'-nuclease under isothermal conditions. The analyte serves as recognition element enable cleave provide (i) first fragment that substantially non-hybridizable and (ii) second lies 3' (in intact oligonucleotide) hybridizable At least 100-fold molar excess and/or are obtained relative amount detected, thereof indicating particular such DNA. Kits conducting methods accordance present invention also disclosed.