[14] Yeast Spheroplasts

摘要: Publisher Summary Yeast cells are treated with a mercaptan compound and EDTA, whereby the cell wall is made more sensitive to attack of lytic enzymes. The then digested snail gut juice. An osmotic stabilizer must be present during incubation prevent bursting spheroplasts. pretreatment SH compounds EDTA greatly facilitates subsequent by Cells from stationary phase converted completely appropriate amount enzyme ascertained for each strain yeast. For logarithmic Saccharomyces carlsbergensis ( S. ), volume corresponding about 0.3 ml original “glusulase” usually sufficient convert 1 g yeast (wet weight) into spheroplasts in 45 minutes. spheroplast preparation may contaminated numerous bud scars, at least if used as source scars consist mainly chitin resistant “glusulase,” which has only weak chitinase activity.