Cell lysis and protein extraction in a microfluidic device with detection by a fluorogenic enzyme assay.

摘要: A critical requirement for achieving a micro total analytical system the analysis of cells and their constituent proteins is to integrate lysis fractionation steps on-chip. Here, an experimental microfluidic integrating bacterial extraction large intracellular enzyme, β-galactosidase, demonstrated. The β-galactosidase detected quantified using fluorogenic enzyme assay numerical model. While focus on typical Gram-negative (E. coli), techniques described here could, in principle, be applied variety different cell types.