Suppression of Plant Immune Responses by the Pseudomonas savastanoi pv. savastanoi NCPPB 3335 Type III Effector Tyrosine Phosphatases HopAO1 and HopAO2.

摘要: The effector repertoire of the olive pathogen P. savastanoi pv. NCPPB 3335 includes two members HopAO family, one most diverse T3E families syringae complex. study described here explores phylogeny these dissimilar members, HopAO1 and HopAO2, among complex reveals their activities as immune defence suppressors. Although is predominantly encoded by phylogroup 3 strains isolated from woody organs hosts, both HopAO2 are phylogenetically clustered according to woody/herbaceous nature host isolation, suggesting specialization family across translocate into plant cells show hrpL-dependent expression, which allows classification actively deployed type III effectors. Our data also that possess phosphatase activity, a hallmark this family. Both them exert an inhibitory effect on early responses, such ROS production callose deposition, able suppress ETI responses induced effectorless polymutant tomato DC3000 (DC3000D28E) in Nicotiana. Moreover, we demonstrate ΔhopAO1 mutant NCPBB exhibits reduced fitness virulence plants, supports relevance during interaction strain with its plants. This work contributes field first report regarding functional analysis homologs or hosts.