Toward the bilayer proteome, electrospray ionization-mass spectrometry of large, intact transmembrane proteins
作者： J. P. Whitelegge , J. le Coutre , J. C. Lee , C. K. Engel , G. G. Prive
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摘要: Genes encoding membrane proteins comprise a substantial proportion of genomes sequenced to date, but ability perform structural studies on this portion the proteome is limited. Electrospray ionization-MS (ESI-MS) an intact protein generates profile defining native covalent state gene product and its heterogeneity. Here we apply ESI-MS technology with accuracy exceeding 0.01% hydrophobic 12-transmembrane α-helices, full-length lactose permease from Escherichia coli. Furthermore, used titrate reactive thiols N-ethylmaleimide. Treatment solubilized in detergent micelles reveals only two thiols, both are protected by substrate analog.
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