Expression of Inducible Nitric Oxide in Human Lung Epithelial Cells

摘要: Nitric oxide (NO) is increased in the exhaled air of subjects with several airway disorders. To determine if cytokines could stimulate epithelial cells accounting for NO, capacity proinflammatory (cytomix: tumor necrosis factor-alpha, interleukin-1 beta, and interferon-gamma) to increase inducible nitric synthase (iNOS) was investigated A549 primary cultures human bronchial cells. Cytomix induced a time-dependent nitrite levels culture supernatant fluids (p < 0.05). Increased numbers stained iNOS mRNA detected cytokine-stimulated compared control Dexamethasone diminished cytokine-induced nitrite, by immunocytochemistry, mRNA. These data demonstrate that cytokines, such as those released mononuclear cells, can induce lung expression NO release, this attenuated dexamethasone.