Regulation of inhibin subunit gene expression by FSH and estradiol in cultured rat granulosa cells

摘要: Abstract Roles of follicle-stimulating hormone (FSH) and sex steroids in regulating the expression mRNA species encoding α-, βA- βBsubunits inhibin were studied cultured granulosa cells from immature rat ovaries. Inhibin subunit mRNAs detected by Northern blot analysis total RNA extracted cell monolayers which had been incubated for 48 h serum-free medium containing FSH (100 ng/ml) and/or a steroid (10−6 M): estradiol (E), testosterone (T) or 5ct-dihydrotestosterone (DHT). Levels each untreated (control) cultures low. In treated with alone, levels ββ-subunit approximately 60-fold, 70-fold 66-fold greater than control, respectively. E a- βB-subunit elevated 4-fold 2-fold, respectively, but level βA-subunit was not measurably affected. Treatment T DHT alone no con...