Biofabrication using maize protein: 3D printing using zein formulations

摘要: Abstract The use of three-dimensional (3D) printing for biomedical applications has expanded exponentially in recent years. However, the current portfolio 3D printable inks is still limited. For instance, only a few protein matrices have been explored as printing/bioprinting materials. Here, we introduce zein, primary constitutive maize seeds, 3D-printable material. Zein-based were prepared by dissolving commercial zein powder ethanol with or without polyethylene glycol (PEG400) plasticizer. rheological characteristics our materials, studied during 21 days aging/maturation, showed an increase apparent viscosity function time all formulations. addition PEG 400 decreased viscosity. Inks and PEG400 at different maturation times tested printability BioX bioprinter. We optimized parameters each ink formulation terms extrusion pressure linear velocity. Higher fidelity structures obtained that had 10 to 14 days. present proof-of-concept experiments demonstrate versatility engineered diverse applications. These include complex and/or free-standing structures, materials controlled drug release, scaffolds cell culture.