Phosphatidic acid distribution on the external surface of mixed vesicles.

摘要: A new method has been developed to detect the distribution of phosphatidic acid on external surface mixed phospholipid vesicles. Some positive dyes undergo large absorbance changes when spatial separation between two or more dye molecules is smaller than a critical distance. When these interact with vesicles, may be utilized calculate amount which, surface, occupy nearby positions not exceeding distance, i.e., paired molecules. This was found higher that calculated by statistical methods, indicating tend associated, in spite electrostatic repulsion negative groups. The dependence pH, phosphatidylcholine:phosphatidic ratio, and temperature also analyzed.