Activation of Hedgehog Signaling Inhibits Osteoblast Differentiation of Human Mesenchymal Stem Cells

摘要: Mesenchymal stem cells within the bone are responsible for generation of osteoblasts, chondrocytes, and adipocytes. In rodents, Indian hedgehog has been shown to play a role in osteoblast differentiation. However, evidence direct function (Hh) human osteoblastic differentiation is missing. Using different models mesenchymal we show that Hh signaling decreases during This associated with decrease Smoothened expression, key partner triggers signaling, number displaying primary cilium, an organelle necessary signaling. Remarkably, treatment sonic or two molecules able activate inhibits inhibition visualized through mineralization expression genes. particular, activation induces Runx2 transcriptional factor controlling early stage Consistently, first days sufficient inhibit differentiation, whereas differentiated osteoblasts not affected by summary, here, using various inducers origins, sharp contrast what described rodent cells. species difference should be taken into account screening pro-osteogenic molecules.