Promoter Trapping in Plants Using T-DNA Mutagenesis

摘要: Promoter is a DNA fragment with crucial cis-acting signature sequences governing the transcription of an adjoining gene. The promoter elements govern level expression associated gene, determine responsiveness gene to physical and/or chemical stimuli and decide whether would constitutively express or exhibit tissue developmental stage specific expression. Depending upon signal present in element respond one combination more than such signal. thus key component for regulated introduced genes transgenic plants. A range being identified exploited need-based tailoring transgenes both basic applied purposes. variety approaches are employed isolate from plants, amongst them transferred (T-DNA) insertion mutagenesis has been extensively used identification cloning genes, promoters, enhancers other regulatory sequences. In this chapter, we discuss T-DNA based approach isolation We also highlight impact tagging strategy functional genomics studies some recent developments field cloning, characterization Arabidopsis thaliana. Finally, summarize commonly analysis plant promoters.