The Effect of Various Inulins and Clostridium difficile on the Metabolic Activity of the Human Colonic Microbiota in vitro

摘要: The influence of inulins with different average degree polymerization (ranging from 3 to 25) on the metabolic activity human colonic microbiota or without addition Clostridium difficile was investigated in vitro. vitro system used a dynamic, computer-controlled model that simulates conditions proximal part large intestine peristaltic mixing, water absorption and fermentation products. inulin stimulated formation total amount short-chain fatty acids acetate, propionate butyrate up 50%, lactate > 10-fold for inulin, while ammonia branched-chain iso-butyrate iso-valerate suppressed. Ammonia suppressed by about 30% almost completely These effects became much more pronounced when C. present system. introduction caused stimulation production protein fermentative metabolites ammonia, phenolic compounds indole, phenol p-cresol. This stimulatory effect prevented inulins. Thus, these results indicate potential shift towards less potentially toxic metabolites, both under normal disturbed (with high level difficile).