ADAMTS-4 (aggrecanase-1): N-terminal activation mechanisms.

摘要: Abstract ADAMTS-4 (aggrecanase 1) is synthesized as a latent precursor protein that may require activation through removal of its prodomain before it can exert catalytic activity. We examined various proteinases well auto-activation under wide range conditions for the and induction enzymatic The proprotein convertases, furin, PACE4, PC5/6 efficiently removed cleavage at Arg 212 /Phe 213 , generating an active enzyme. Of broad proteases evaluated, only MMP-9 trypsin were capable removing prodomain. In presence mercuric compounds, autocatalysis was not observed, nor observed temperatures from 22 to 65 °C, ionic strengths 0.1 1 M, or acidic/neutral pH. At basic pH 8–10, by occurred, conclusion, pro-form catalytically limited number mechanisms mediate N-terminal activation.