The release of growth arrest by microinjection of adenovirus E1A DNA.

摘要: Abstract The induction of DNA synthesis in growth-arrested mouse fibroblasts (NIH 3T3) was studied by microinjection different constructs adenovirus using SV40 and plasmid as positive negative controls. The E1A region types 2 12 appears to be sufficient induce cellular after growth arrest approximately 30% the cells both 13S 12S cDNA mediate this effect. presence protein products assayed immunofluorescence does not strictly correlate with microinjected contrast large T-antigen. Microinjection truncated fragments Ad12 suggests, however, that may involved process. A sequence comparison T-antigen identified a significant homology providing basis for hypothesis concerning evolution genes viruses.