Thermodynamics of ligand-nucleic acid interactions.

摘要: Ligand-and protein-DNA equilibria are extremely sensitive to solution conditions (e.g., salt, temperature, and pH), and, in general, the effects of different variables interdependent (i.e., linked). As a result, an assessment basis for stability specificity ligand-or interactions requires quantitative studies these as function range variables. Many most dramatic on result from changes entropy system, caused by preferential interaction small molecules, principally ions which released into complex formation. A determination contributions protein-and ligand-DNA thermodynamic approaches cannot be assessed structural alone.