作者: P. A. Lalley , U. Francke , J. D. Minna
关键词:
摘要: Abstract It is possible to generate interspecific somatic cell hybrids that preferentially segregate mouse chromosomes, thus making mapping of genes. Therefore, comparison the linkage relationships homologous genes in man and now possible. Chinese hamster × segregating chromosomes were tested for expression enolase (ENO-1; EC 4.2.1.11, McKusick no. 17245), 6-phosphogluconate dehydrogenase [PGD; 1.1.1.44, 17220], phosphoglucomutase-2 (PGM-2; 2.7.5.1, 17190), adenylate kinase-2 (AK-2; 2.7.4.3, 10302). In man, coding forms these enzymes have been assigned short arm human chromosome 1. Analysis 41 primary, independent, hybrid clones indicated that, mouse, ENO-1 AK-2 are syntenic with PGD PGM-2 therefore can be 4. contrast, they asyntenic 21 other including dipeptidase-1 (DIP-1, PEP-C; 3.4.11.*, 17000) 1q Karyologic analysis confirmed this assignment. These data demonstrate a large autosomal region (21 map units 51 male) has conserved evolution 4 Identification such regions will contribute our understanding mammalian genome could suggest gene location by homology mapping.