Isolation of lipid droplets from cells by density gradient centrifugation.
作者： Dawn L. Brasaemle , Nathan E. Wolins
DOI: 10.1002/CPCB.10
关键词:
摘要: … Transfer the homogenate to a 15-ml tube. … by additional wash steps; transfer the lipid droplet fraction to a microcentrifuge tube, centrifuge 10 min at maximum speed at 4C, and remove …
sci-hub.se 参考文章(29)
John M. Graham, Purification of a Crude Mitochondrial Fraction by Density‐Gradient Centrifugation Current protocols in pharmacology. ,vol. 4, ,(1999) , 10.1002/0471143030.CB0304S04
D L Brasaemle, T Barber, N E Wolins, G Serrero, E J Blanchette-Mackie, C Londos, Adipose differentiation-related protein is an ubiquitously expressed lipid storage droplet-associated protein. Journal of Lipid Research. ,vol. 38, pp. 2249- 2263 ,(1997) , 10.1016/S0022-2275(20)34939-7
Peter M. Gocze, Dale A. Freeman, Factors underlying the variability of lipid droplet fluorescence in MA-10 leydig tumor cells† Cytometry. ,vol. 17, pp. 151- 158 ,(1994) , 10.1002/CYTO.990170207
Ellen Umlauf, Edina Csaszar, Manuel Moertelmaier, Gerhard J. Schuetz, Robert G. Parton, Rainer Prohaska, Association of Stomatin with Lipid Bodies Journal of Biological Chemistry. ,vol. 279, pp. 23699- 23709 ,(2004) , 10.1074/JBC.M310546200
Dawn L. Brasaemle, Therese Barber, Alan R. Kimmel, Constantine Londos, Post-translational Regulation of Perilipin Expression STABILIZATION BY STORED INTRACELLULAR NEUTRAL LIPIDS Journal of Biological Chemistry. ,vol. 272, pp. 9378- 9387 ,(1997) , 10.1074/JBC.272.14.9378
Isabelle Petitpas, Tim Grüne, Ananyo A Bhattacharya, Stephen Curry, Crystal structures of human serum albumin complexed with monounsaturated and polyunsaturated fatty acids Journal of Molecular Biology. ,vol. 314, pp. 955- 960 ,(2001) , 10.1006/JMBI.2000.5208
Pingsheng Liu, Yunshu Ying, Yingming Zhao, Dorothy I. Mundy, Meifang Zhu, Richard G. W. Anderson, Chinese hamster ovary K2 cell lipid droplets appear to be metabolic organelles involved in membrane traffic. Journal of Biological Chemistry. ,vol. 279, pp. 3787- 3792 ,(2004) , 10.1074/JBC.M311945200
Masato Ohashi, Noboru Mizushima, Yukiko Kabeya, Tamotsu Yoshimori, Localization of mammalian NAD(P)H steroid dehydrogenase-like protein on lipid droplets. Journal of Biological Chemistry. ,vol. 278, pp. 36819- 36829 ,(2003) , 10.1074/JBC.M301408200
Dawn L. Brasaemle, Georgia Dolios, Lawrence Shapiro, Rong Wang, Proteomic Analysis of Proteins Associated with Lipid Droplets of Basal and Lipolytically Stimulated 3T3-L1 Adipocytes * Journal of Biological Chemistry. ,vol. 279, pp. 46835- 46842 ,(2004) , 10.1074/JBC.M409340200
H. Caldas, NSDHL, an enzyme involved in cholesterol biosynthesis, traffics through the Golgi and accumulates on ER membranes and on the surface of lipid droplets Human Molecular Genetics. ,vol. 12, pp. 2981- 2991 ,(2003) , 10.1093/HMG/DDG321