[Protein engineering: from directed evolution to computational design].

摘要: By constructing mutant libraries and utilizing high-throughput screening methods, directed evolution has emerged as the most popular strategy for protein design nowadays. In past decade, taking advantages of computer performance algorithms, computer-assisted rapidly developed become a powerful method engineering. Based on simulation structure calculation energy function, computational can alter substrate specificity improve thermostability enzymes, well de novo artificial enzymes with expected functions. Recently, machine learning other intelligence technologies have also been applied to engineering, resulting in series remarkable applications. Along lines this paper reviews progress applications design, current trends outlooks development.