Robustness in glyoxylate bypass regulation.

摘要: The glyoxylate bypass allows Escherichia coli to grow on carbon sources with only two carbons by bypassing the loss of as CO2 in tricarboxylic acid cycle. flux toward this is regulated phosphorylation enzyme isocitrate dehydrogenase (IDH) a bifunctional kinase–phosphatase called IDHKP. In system, IDH activity has been found be remarkably robust respect wide variations total protein concentration. Here, we examine possible mechanisms explain robustness. Explanations which IDHKP works simultaneously first-order kinase and zero-order phosphatase single binding site are inconsistent Instead, suggest mechanism where both substrates bind form ternary complex.