Patient-specific embryonic stem cells derived from human SCNT blastocysts.
作者： Woo Suk Hwang , Sung Il Roh , Byeong Chun Lee , Sung Keun Kang , Dae Kee Kwon
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摘要: Patient-specific, immune-matched human embryonic stem cells (hESCs) are anticipated to be of great biomedical importance for studies disease and development advance clinical deliberations regarding cell transplantation. Eleven hESC lines were established by somatic nuclear transfer (SCNT) skin from patients with or injury into donated oocytes. These lines, (NT)-hESCs, grown on feeders the same NT donor genetically unrelated individuals, at high rates, regardless sex age. NT-hESCs pluripotent, chromosomally normal, matched patient's DNA. The major histocompatibility complex identity each NT-hESC when compared own showed immunological compatibility, which is important eventual With generation these NT-hESCs, evaluations genetic epigenetic stability can made. Additional work remains done reliable directed differentiation elimination remaining animal components. Before use occur, preclinical evidence required prove that transplantation differentiated safe, effective, tolerated.
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