Effects of intestinal ischemia reperfusion injury on the level of specific genes in rats
作者： M. Bilecová-Rabajdová , S. Čikoš , J. Veselá , S. Czikková , K. Gregová
DOI:
关键词:
摘要: The small intestine is an organ with very well developed immunological activity. There are specific cells in the mucosa of responsible for releasing inflammatory mediators that can lead to Multiple Organ Dysfunction Syndrome (MODS), which a complex process occur after ischemia-reperfusion injury. accumulation wall also increases expression apoptotic genes. aim this study was detect and analyse changes genes (Bax, Bcl2) production cytokines (TNFα, IL1β, IL6, IL10) tumour growth factor beta (TGFβ). Male Wistar rats underwent ischemia performed by complete occlusion mesenteric artery. Ischemia followed reperfusion periods 1 hour, 24 hours, 30 days. Subsequently, total RNA isolated from RT-PCR (real-time) performed. significant increase levels Bcl2, TNFα, IL10, TGFβ) one hour decreased tendency hours Streszczenie:
wsew.edu.pl 参考文章(12)
F J Geske, R Lieberman, R Strange, L E Gerschenson, Early stages of p53-induced apoptosis are reversible Cell Death & Differentiation. ,vol. 8, pp. 182- 191 ,(2001) , 10.1038/SJ.CDD.4400786
Santos A. Susin, Naoufal Zamzami, Guido Kroemer, Mitochondria as regulators of apoptosis: doubt no more Biochimica et Biophysica Acta. ,vol. 1366, pp. 151- 165 ,(1998) , 10.1016/S0005-2728(98)00110-8
Li Yang, Harold L. Moses, Transforming Growth Factor β: Tumor Suppressor or Promoter? Are Host Immune Cells the Answer? Cancer Research. ,vol. 68, pp. 9107- 9111 ,(2008) , 10.1158/0008-5472.CAN-08-2556
Martin R.W. Grotz, Edwin A. Deitch, Jiayi Ding, Dazhong Xu, Qinghong Huang, Gerd Regel, Intestinal cytokine response after gut ischemia: role of gut barrier failure. Annals of Surgery. ,vol. 229, pp. 478- 486 ,(1999) , 10.1097/00000658-199904000-00005
P. Urban, M. Bilecova-Rabajdova, M. Marekova, J. Vesela, Progression of apoptic signaling from mesenteric ischemia-reperfusion injury to lungs: correlation in the level of ER chaperones expression. Molecular and Cellular Biochemistry. ,vol. 362, pp. 133- 140 ,(2012) , 10.1007/S11010-011-1135-4
A. Stallion, T.D. Kou, D.S. Berger, K.A. Miller, D.L. Dudgeon, A.D. Levine, Intestinal production of IL-6 initiates systemic inflammation in a murine model of intestinal ischemia reperfusion1 Current Surgery. ,vol. 56, pp. 384- 385 ,(1999) , 10.1016/S0149-7944(99)00141-5
B Zingarelli, Z Yang, PW Hake, A Denenberg, HR Wong, Absence of endogenous interleukin 10 enhances early stress response during post-ischaemic injury in mice intestine Gut. ,vol. 48, pp. 610- 622 ,(2001) , 10.1136/GUT.48.5.610
Kei Higashikawa, Naoki Akada, Katsuharu Yagi, Keiko Watanabe, Shinichiro Kamino, Yousuke Kanayama, Makoto Hiromura, Shuichi Enomoto, Exploration of target molecules for molecular imaging of inflammatory bowel disease. Biochemical and Biophysical Research Communications. ,vol. 410, pp. 416- 421 ,(2011) , 10.1016/J.BBRC.2011.05.146
A Deten, Cardiac cytokine expression is upregulated in the acute phase after myocardial infarction. Experimental studies in rats. Cardiovascular Research. ,vol. 55, pp. 329- 340 ,(2002) , 10.1016/S0008-6363(02)00413-3
G. S. Salvesen, V. M. Dixit, Caspase activation: the induced-proximity model. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 96, pp. 10964- 10967 ,(1999) , 10.1073/PNAS.96.20.10964