ECDI-fixed allogeneic splenocytes induce donor-specific tolerance for long-term survival of islet transplants via two distinct mechanisms

摘要: A major challenge for human allogeneic islet transplantation is the development of effective methods to induce donor-specific tolerance obviate need life-long immunosuppression that toxic insulin-producing β cells and detrimental host. We developed an efficient therapy utilizes infusions ethylene carbodiimide (ECDI)-treated donor splenic antigen-presenting results in indefinite survival grafts absence immunosuppression. Furthermore, we show induction critically dependent on synergistic effects between intact programmed death 1 receptor–programmed ligand signaling pathway CD4+CD25+Foxp3+ regulatory T cells. This highly antigen-specific with a complete avoidance has significant therapeutic potential cell transplantation.