Field Validity and Feasibility of Four Techniques for the Detection of Trichuris in Simians: A Model for Monitoring Drug Efficacy in Public Health?

摘要: Background: Soil-transmitted helminths, such as Trichuris trichiura, are of major concern in public health. Current efforts to control these helminth infections involve periodic mass treatment endemic areas. Since large-scale interventions likely intensify, monitoring the drug efficacy will become indispensible. However, studies comparing detection techniques based on sensitivity, fecal egg counts (FEC), feasibility for diagnosis and estimates scarce. Methodology/Principal Findings: In present study, ether-based concentration, Parasep Solvent Free (SF), McMaster FLOTAC were compared both validity eggs 100 samples nonhuman primates. addition, quantitative was examined using a statistical simulation. found 47% samples. most sensitive technique (100%), followed by SF (83.0% [95% confidence interval (CI): 82.4-83.6%]) concentration (76.6% [ 95% CI: 75.8-77.3%]). least (61.7% 60.7-62.6%]) failed detect low FEC. The comparison revealed positive correlation between four (Rs = 0.85-0.93; p < 0.0001). detected significantly fewer than (p 0.0083). Overall, feasible (3.9 min/sample preparing, reading cleaning apparatus), (7.7 min/sample) (9.8 min/sample). (17.7 min/ sample). simulation that sensitivity is less important reliable estimators. Conclusions/Significance: results this study demonstrated promising when making use FEC monitor Trichuris.