Brief Report: A Bioassay to Identify Primary Human Prostate Cancer Repopulating Cells

摘要: Cancer cells are heterogeneous in both their phenotypes and ability to promote tumor growth spread. Xenografting is used identify the most highly capable of regenerating tumors, referred as cancer repopulating cells. Because prostate cancers (PCa's) rarely grow xenografts, indentifying PCa has not been possible. Here, we report improved methods xenograft localized primary tissues using chimeric grafts with neonatal mouse mesenchyme. Xenograft survival tissue was significantly increased by mesenchyme (six six patients, 66% grafts, versus four 41% grafts) doubled proliferation index xenografted When applied isolated cells, effectively reconstituted PCa's (four nine patients; 32% 0% without), supported active cell proliferation. Using this assay, showed that unfractionated α2β1integrin(hi) α2β1integrin(lo) from demonstrated formation at comparable rates, similar previous reports metastatic specimens. Thus, new protocol efficiently established tumors enabled proliferative expansion intact fractionated providing a bioassay therapeutically target