Analysis of detergent-resistant membranes of Helicobacter pylori infected gastric adenocarcinoma cells reveals a role for MARK2/Par1b in CagA-mediated disruption of cellular polarity.
作者： Antonello Covacci , Markus Stein , Zaher Zeaiter , David Cohen , Anne Müsch
DOI: 10.1111/J.1462-5822.2007.01084.X
关键词:
摘要: Summary Detergent-resistant membranes of eukaryotic cells are enriched in many important cellular signalling molecules and frequently targeted by bacterial pathogens. To learn more about pathogenic mechanisms Helicobacter pylori to elucidate novel effects on host epithelial cells, we investigated how co-cultivation changes the protein composition detergent-resistant gastric adenocarcinoma (AGS) tissue culture cells. Using iTRAQ (isobaric tags for relative absolute quantification) analysis identified several proteins, which potentially related H. virulence. One showed a significant infection-dependent increase detergent resistance, was polarity-associated serine/threonine kinase MARK2 (EMK1/Par-1b). We demonstrate that causes recruitment from cytosol plasma membrane, where it colocalizes with bacteria interacts CagA. Mardin Darby Canine Kidney (MDCK) monolayers three-dimensional MDCK model association CagA not only disruption apical junctions, but also inhibition tubulogenesis cell differentiation.
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