Cofactor-specific Anchoring of Horseradish Peroxidase onto a Polythiophene-modified Electrode
作者： Akira Onoda , Yasunari Umeda , Takashi Hayashi
DOI: 10.1246/CL.170837
关键词:
摘要: Horseradish peroxidase apoprotein (apoHRP) was immobilized on a heme-modified polythiophene film, which electropolymerized an indium tin oxide surface to yield and reconstituted HRP. The HRP-immobilized electrode exhibits 6-fold enhanced electrochemical response toward hydrogen peroxide reduction relative having the protein randomly electrode. This indicates that insertion of heme moiety into pocket HRP leads increase in electrocatalytic current.
csj.jp参考文章(51)
Bioelectronics : from theory to applications Wiley-VCH Verlag GmbH & Co. KGaA. ,(2005) , 10.1002/352760376X
Bertrand Reuillard, Solène Gentil, Marie Carrière, Alan Le Goff, Serge Cosnier, Biomimetic versus enzymatic high-potential electrocatalytic reduction of hydrogen peroxide on a functionalized carbon nanotube electrode Chemical Science. ,vol. 6, pp. 5139- 5143 ,(2015) , 10.1039/C5SC01473E
Alaa-Eldin F Nassar, William S Willis, James F Rusling, None, Electron transfer from electrodes to myoglobin: facilitated in surfactant films and blocked by adsorbed biomacromolecules. Analytical Chemistry. ,vol. 67, pp. 2386- 2392 ,(1995) , 10.1021/AC00110A010
Liang-Hong Guo, George McLendon, Haja Razafitrimo, Yongli Gao, Photo-active and electro-active protein films prepared by reconstitution with metalloporphyrins self-assembled on gold Journal of Materials Chemistry. ,vol. 6, pp. 369- 374 ,(1996) , 10.1039/JM9960600369
Koji Oohora, Sabina Burazerovic, Akira Onoda, Yvonne M. Wilson, Thomas R. Ward, Takashi Hayashi, Chemically programmed supramolecular assembly of hemoprotein and streptavidin with alternating alignment. Angewandte Chemie. ,vol. 51, pp. 3818- 3821 ,(2012) , 10.1002/ANIE.201107067
Akira Onoda, Yasuaki Kakikura, Taro Uematsu, Susumu Kuwabata, Takashi Hayashi, Photocurrent Generation from Hierarchical Zinc‐Substituted Hemoprotein Assemblies Immobilized on a Gold Electrode Angewandte Chemie. ,vol. 51, pp. 2628- 2631 ,(2012) , 10.1002/ANIE.201105186
Claudia Ley, Dirk Holtmann, Klaus-Michael Mangold, Jens Schrader, Immobilization of histidine-tagged proteins on electrodes Colloids and Surfaces B: Biointerfaces. ,vol. 88, pp. 539- 551 ,(2011) , 10.1016/J.COLSURFB.2011.07.044
Nikitas Bistolas, Ulla Wollenberger, Christiane Jung, Frieder W. Scheller, Cytochrome P450 biosensors-a review. Biosensors and Bioelectronics. ,vol. 20, pp. 2408- 2423 ,(2005) , 10.1016/J.BIOS.2004.11.023
Guo-Xian Ma, Tian-Hong Lu, Yong-Yao Xia, Direct electrochemistry and bioelectrocatalysis of hemoglobin immobilized on carbon black Bioelectrochemistry. ,vol. 71, pp. 180- 185 ,(2007) , 10.1016/J.BIOELECHEM.2007.04.002
Yunhua Wu, Shengshui Hu, Biosensors based on direct electron transfer in redox proteins Mikrochimica Acta. ,vol. 159, pp. 1- 17 ,(2007) , 10.1007/S00604-007-0749-4