Decrease or increase in cardiac muscarinic cholinergic receptor number in rats treated with methacholine or atropine
作者： Bradley C. Wise , Mamoru Shoji , J.F. Kuo
DOI: 10.1016/0006-291X(80)90405-2
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摘要: The effects of chronic treatment the rat with methacholine and atropine on cardiac muscarinic cholinergic receptors were investigated. [3H]Quinuclidinyl benzilate ([3H]QNB) was used to directly estimate number affinity in heart ventricular membrane. Methacholine decreased, a dose-related time-dependent manner, specific binding [3H]QNB by 34% as compared control. Atropine treatment, other hand, resulted increase (28 66%) receptors. equilibrium dissociation constant (KD) for ligand same (about 200 pM) control treated groups rats, whereas (39 105%) KD noted rats. Similarly, concentration acetylcholine causing 50 percent inhibition (IC50) unaltered rats (4 μM), but it increased 43%
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sci-hub.se 参考文章(15)
D. Cavey, J.P. Vincent, M. Làzdunski, The muscarinic receptor of heart cell membranes Association with agonists, antagonists and antiarrhythmic agents FEBS Letters. ,vol. 84, pp. 110- 114 ,(1977) , 10.1016/0014-5793(77)81068-5
N D Goldberg, M K Haddox, Cyclic GMP Metabolism and Involvement in Biological Regulation Annual Review of Biochemistry. ,vol. 46, pp. 823- 896 ,(1977) , 10.1146/ANNUREV.BI.46.070177.004135
Henry I. Yamamura, Solomon H. Snyder, Muscarinic Cholinergic Receptor Binding in the Longitudinal Muscle of the Guinea Pig Ileum with [3H]Quinuclidinyl Benzilate Molecular Pharmacology. ,vol. 10, pp. 861- 867 ,(1974)
Beverly H. Dulis, Irwin B. Wilson, Michael A. Gordon, Identification of muscarinic binding sites in human neutrophils by direct binding. Molecular Pharmacology. ,vol. 15, pp. 28- 34 ,(1979)
Pedro Cuatrecasas, Morley D. Hollenberg, Membrane receptors and hormone action. Advances in Protein Chemistry. ,vol. 30, pp. 251- 451 ,(1976) , 10.1016/S0065-3233(08)60481-7
L T Williams, R J Lefkowitz, A M Watanabe, D R Hathaway, H R Besch, Thyroid hormone regulation of beta-adrenergic receptor number. Journal of Biological Chemistry. ,vol. 252, pp. 2787- 2789 ,(1977) , 10.1016/S0021-9258(17)40528-X
J.B. Galper, W. Klein, W.A. Catterall, Muscarinic acetylcholine receptors in developing chick heart. Journal of Biological Chemistry. ,vol. 252, pp. 8692- 8699 ,(1977) , 10.1016/S0021-9258(19)75276-4
Jyh-Fa Kuo, Tee-Ping Lee, Procerfina L. Reyes, Kenneth G. Walton, Thomas E. Donnelly, Paul Greengard, Cyclic Nucleotide-dependent Protein Kinases X. AN ASSAY METHOD FOR THE MEASUREMENT OF GUANOSINE 3',5'-MONOPHOSPHATE IN VARIOUS BIOLOGICAL MATERIALS AND A STUDY OF AGENTS REGULATING ITS LEVELS IN HEART AND BRAIN Journal of Biological Chemistry. ,vol. 247, pp. 16- 22 ,(1972) , 10.1016/S0021-9258(19)45752-9
J.Z. Fields, W.R. Roeske, E. Morkin, H.I. Yamamura, Cardiac muscarinic cholinergic receptors. Biochemical identification and characterization. Journal of Biological Chemistry. ,vol. 253, pp. 3251- 3258 ,(1978) , 10.1016/S0021-9258(17)40829-5
V.K. Sharma, S.P. Banerjee, Muscarinic cholinergic receptors in rat heart. Effects of thyroidectomy. Journal of Biological Chemistry. ,vol. 252, pp. 7444- 7446 ,(1977) , 10.1016/S0021-9258(17)40985-9