Direct Heme Transfer Reactions in the Group A Streptococcus Heme Acquisition Pathway
作者： Chunmei Lu , Gang Xie , Mengyao Liu , Hui Zhu , Benfang Lei
DOI: 10.1371/JOURNAL.PONE.0037556
关键词:
摘要: The heme acquisition machinery in Group A Streptococcus (GAS) consists of the surface proteins Shr and Shp ATP-binding cassette transporter HtsABC. cannot directly acquire from methemoglobin (metHb) but transfers its to HtsA. It has not been previously determined whether relays metHb Shp. Thus, complete pathway for by GAS remained unclear. In this study, metHb-to-Shr Shr-to-Shp transfer reactions were characterized spectroscopy, kinetics protein-protein interaction analyses. Heme is efficiently transferred β α subunits with rates that are 7 60 times greater than those passive release metHb, indicating acquires metHb. rapid involves an initial donor/acceptor complex a spectrally kinetically detectable intermediate, implying channeled present results show speeds up Shp, whereas Furthermore, findings demonstrate can interact Taken together our published on Shp/HtsA reaction, these establish model which extracts it
harvard.edu
montana.edu
paperity.org
researchgate.net 参考文章(31)
Phillip E. Klebba, Jeanette M. Rutz, Christopher K. Murphy, Jun Liu, Mechanisms of TonB-catalyzed iron transport through the enteric bacterial cell envelope. Journal of Bioenergetics and Biomembranes. ,vol. 25, pp. 603- 611 ,(1993) , 10.1007/BF00770247
Franca Ascoli, Maria Rosaria Rossi Fanelli, Eraldo Antonini, [5] Preparation and properties of apohemoglobin and reconstituted hemoglobins Methods in Enzymology. ,vol. 76, pp. 72- 87 ,(1981) , 10.1016/0076-6879(81)76115-9
Mahamoudou Ouattara, Elizabeth Bentley Cunha, Xueru Li, Ya‐Shu Huang, Dabney Dixon, Zehava Eichenbaum, None, Shr of group A streptococcus is a new type of composite NEAT protein involved in sequestering haem from methaemoglobin Molecular Microbiology. ,vol. 78, pp. 739- 756 ,(2010) , 10.1111/J.1365-2958.2010.07367.X
Tyler K Nygaard, Mengyao Liu, Michael J McClure, Benfang Lei, Identification and characterization of the heme-binding proteins SeShp and SeHtsA of Streptococcus equi subspecies equi BMC Microbiology. ,vol. 6, pp. 82- 82 ,(2006) , 10.1186/1471-2180-6-82
Mark S Hargrove, Eileen W Singleton, Michael L Quillin, Luis A Ortiz, George N Phillips Jr, John S Olson, Antony J Mathews, His64(E7)→Tyr apomyoglobin as a reagent for measuring rates of hemin dissociation Journal of Biological Chemistry. ,vol. 269, pp. 4207- 4214 ,(1994) , 10.2210/PDB1MGN/PDB
Kimberly A. Burkhard, Angela Wilks, Characterization of the Outer Membrane Receptor ShuA from the Heme Uptake System of Shigella dysenteriae SUBSTRATE SPECIFICITY AND IDENTIFICATION OF THE HEME PROTEIN LIGANDS Journal of Biological Chemistry. ,vol. 282, pp. 15126- 15136 ,(2007) , 10.1074/JBC.M611121200
Erin Sarah Honsa, Marian Fabian, Ana Maria Cardenas, John S. Olson, Anthony William Maresso, The Five Near-iron Transporter (NEAT) Domain Anthrax Hemophore, IsdX2, Scavenges Heme from Hemoglobin and Transfers Heme to the Surface Protein IsdC Journal of Biological Chemistry. ,vol. 286, pp. 33652- 33660 ,(2011) , 10.1074/JBC.M111.241687
Sarkis K Mazmanian, Eric P Skaar, Andrew H Gaspar, Munir Humayun, Piotr Gornicki, Joanna Jelenska, Andrzej Joachmiak, Dominique M Missiakas, Olaf Schneewind, Passage of Heme-Iron Across the Envelope of Staphylococcus aureus Science. ,vol. 299, pp. 906- 909 ,(2003) , 10.1126/SCIENCE.1081147
Brian R. Sook, Darci R. Block, Suganya Sumithran, Griselle E. Montañez, Kenton R. Rodgers, John H. Dawson, Zehava Eichenbaum, Dabney W. Dixon, Characterization of SiaA, a streptococcal heme-binding protein associated with a heme ABC transport system. Biochemistry. ,vol. 47, pp. 2678- 2688 ,(2008) , 10.1021/BI701604Y
Yanchao Ran, Mengyao Liu, Hui Zhu, Tyler K. Nygaard, Doreen E. Brown, Marian Fabian, David M. Dooley, Benfang Lei, Spectroscopic Identification of Heme Axial Ligands in HtsA that are Involved in Heme Acquisition by Streptococcus pyogenes Biochemistry. ,vol. 49, pp. 2834- 2842 ,(2010) , 10.1021/BI901987H