Molecular analysis of human- and pig-derived Ascaris in Honduras.

摘要: Ascaris sp. is a soil-transmitted helminth (STH) significantly affecting the health of human and swine populations. Health inequities poverty, with resulting deficiencies in water, sanitation hygiene, are directly associated lumbricoides prevalence humans. Resource constraints also lead to small-scale livestock production under unsanitary conditions. Free-ranging pigs, for instance, exposed number infectious agents, among which suum one most common. Under these conditions, close proximity between people pigs can result cross-contamination; that is, harbouring vice versa. Moreover, potential interbreeding two species has been demonstrated. The present study analysed worms obtained from children Honduras. Adult were collected stool samples after pharmacological treatment, pigs' intestines slaughter commercial purposes at local abattoir. A nuclear ribosomal internal transcribed spacer (ITS) region was amplified by polymerase chain reaction (PCR) digested restriction enzyme order separate putative human- pig-derived isolates. PCR products sequenced, cladograms constructed. All parasites isolated showed typical human-derived genotype Ascaris, whereas 91% expected genotype. Cross-infections hosts not demonstrated this study. Nine per cent band pattern highly suggestive hybrid human-pig These results contribute understanding ascariasis epidemiology its zoonotic endemic region.