Honey bee brood ring-test: method for testing pesticide toxicity on honeybee brood in laboratory conditions

摘要: contribution to session IV Test methodology The Experimental unit of entomology (INRA, France) developed a new in vitro method assess effects pesticides on honey bee larvae. The consists rearing larvae plastic cells. are fed with diet containing 50% fresh royal jelly and an aqueous sugar yeast extract solution, reared incubator at 35 °C 96% relative humidity. According that method, 9 tests (7 2008 2 2005) were carried out 7 laboratories different countries. objective these trials was the LD50 for dimethoate 48 hours after acute exposure. values ranged from 1.5 μg a.i./larva 8.8 a.i./larva, particularly high (5.0 a.i./larva). In tests, 3.1 a.i./larva. Such variability may be due colony origin, season larva heterogeneity grafting. Solutions proposed improve through continuation ring test. Keywords: Apis mellifera, brood, test,