Role of antigen-presenting cells in the cytotoxic T-cell response to minor histocompatibility antigens (MIHA). I. In vitro function of cells pulsed with MIHA in vivo.

摘要: Although antigen-presenting cells (APC) appear to be able process minor histocompatibility antigens (MIHA) expressed on allogeneic and present them in association with intrinsic H-2 of the APC vivo, this does not occur vitro. This could due fundamentally different mechanisms antigen handling by vitro, or it represent compromised function. In order distinguish these possibilities, we designed a system which BALB/c spleen are transferred into an MIHA-disparate irradiated host allowed reside for 2-3 days; repopulated removed used as stimulator CTL response primed responder DBA/2 MIHA. These referred vivo-pulsed (IVP-APC). Donor pick up MIHA after passage hosts efficiently precursors generate DBA/2-specific CTL. The donor cell type is but thymus bone marrow, Thy-1.2 negative, Ia+, nylon wool-adherent. Its stimulatory capacity efficient per basis that cells. generation IVP-APC requires repopulation host, must express foreign When attempted incompatible hosts, found that, although antigens, they were unable antigens. Use intra-H-2 recombinant strains mice indicated compatibility at KI region was essential apparently unprocessed Thus, reproduce antigen-processing function encountering situ using adoptive transfer method pulsing vivo. addition, suggest our results may impose constraints formulation models account