10 – Interfering with Gene Function at the RNA Level with Oligozymes—the Development of New Tools and Therapeutics

摘要: Publisher Summary The hammerhead ribozyme motif, discovered in certain plant pathogens, is a member of small class naturally occurring catalytic ribonucleic acid (RNAs). In nature an intramolecular reaction leads to cis-cleavage; however, it was shown that the motif could be split into two pieces, RNA target sequence and part, cleave trans. Suitable chemical modification has led development oligomers are chemically stable nuclease resistant so they can used cell culture or vivo. Such modified participate sequence-specific cleavage targeted molecule, have been termed oligozymes. The chapter describes classes oligozymes, which one based on ribozymes composed 2'-0-allylribonucleotides. latter analogues possess variety properties render them useful for application molecular biology, notably stability, resistance, strong preference bind over DNA low level nonspecific binding. other oligozyme Innovirs proprietary EGS (External Guide Sequence) technology. oligozymes small, oligonucleotides designed specific sites generate motifs resembling precursor tRNA, cleaved by cellular RNase P.