RTKN2 Induces NF-KappaB Dependent Resistance to Intrinsic Apoptosis in HEK Cells and Regulates BCL-2 Genes in Human CD4+ Lymphocytes:

摘要: The gene for Rhotekin 2 (RTKN2) was originally identified in a promyelocytic cell line resistant to oxysterol-induced apoptosis. It is differentially expressed freshly isolated CD4+ T-cells compared with other hematopoietic cells and down-regulated following activation of the T-cell receptor. However, very little known about function RTKN2 than its homology Rho-GTPase effector, rhotekin, possibility that they may have similar roles. Here we show stable expression HEK enhanced survival response intrinsic apoptotic agents; 25-hydroxy cholesterol camptothecin, but not extrinsic agent, TNFα. Inhibitors NF-KappaB, MAPK, reversed resistance mitochondrial pro-apoptotic genes, Bax Bim, were down regulated. In these cells, there no evidence binding GTPases, RhoA or Rac2. Consistent role over-expressing suppression primary human reduced viability increased sensitivity 25-OHC. Bim while BCL-2 decreased. both models played process apoptosis this dependent on either NF-KappaB signaling downstream genes. As highly it play as key switch regulation genes involved survival.