Hybridoma production by simplified avidin-mediated electrofusion
作者： Don M. Wojchowski , Arthur J. Sytkowski
DOI: 10.1016/0022-1759(86)90073-6
关键词:
摘要: A simplified method for producing cell hybrids by avidin-mediated electrofusion has been developed. First, biotin was attached both to immunogen and the surface of murine myeloma cells using N-hydroxysuccinimidobiotin. Biotinylated incubated with splenocytes derived from immunized mice allowed bind immunoglobulins B cells. Using streptavidin, biotinylated then were bridged those bearing immunogen. Selective fusion accomplished their limited exposure high-voltage potentials. high frequency hybridomas obtained all which secreted titers antibodies a selected model immunogen, keyhole limpet hemocyanin.
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