Alternative Splicing of the Latency-Related Transcript of Bovine Herpesvirus 1 Yields RNAs Containing Unique Open Reading Frames

摘要: The latency-related transcript (LRT) of bovine herpesvirus 1 (BHV-1) is the only abundant viral RNA detected during latency. A previous study (A. Hossain, L. M. Schang, and C. Jones, J. Virol. 69:5345–5352, 1995) concluded that splicing polyadenylated [poly(A)+] nonpolyadenylated [poly(A)−] LRT are different. In this study, splice junction sites were identified. trigeminal ganglia acutely infected calves (1, 7, or 15 days postinfection [p.i.]) in latently (60 p.i.), alternative poly(A)+ occurred. Productive gene expression readily from 2 to 7 p.i. but not at (L. Schang 71:6786–6795, 1997), suggesting certain aspects a lytic infection occur neurons these factors influence splicing. Splicing poly(A)− was also transfected COS-7 cells MDBK cells. DNA sequence analysis spliced cDNAs, poly(A)−, revealed nonconsensus signals exon/intron intron/exon boundaries. GC-AG signal utilized by herpes simplex virus type latency-associated mice used calves. Taken together, results led us hypothesize (i) neuron-specific factors, (ii) virus-induced participate splicing, (iii) may result protein isoforms which have novel biological properties.